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The One Step LH Urine Ovulation Test is fast and
easy-to-use. It is a qualitative test that predicts LH
(Luteinizing Hormone) surge, and in turn, when you are likely
to ovulate and conceive. Females who are trying to become
pregnant may use this information to improve their chances of
conception. Luteinizing Hormone is a hormone released by the
pituitary gland, a small gland at the base of the brain. The
hormone stimulates the ovaries to produce and release eggs
each month during the menstral cycle. The level of LH in the
blood and urine is highest before ovulation. This increase in
hormone level is sometimes called a
"surge". If fertility drugs are given to stimulate ovulation,
an LH test can help determine the best time for sexual
intercourse. The LH test may also be used to determine when
eggs are mature enough to be surgically removed from the ovary
as part of the in vitro fertilization process. LH tests may
also aid in the diagnoses of polycystic ovary disease,
premature ovarian failure, and
menapause.
The tests detect LH in urine in a qualitative format
with sensitivity of 25mIU/mL (International Standard Value)
and specificity is 99.8%. Tests have an expiration date of 1
to 2 years and can be stored in a dry environment ranging from
2 to 30 degrees Celsius. Results are available in just
minutes.
WHEN
TO BEGIN TESTING
First, you must
determine the length of your menstral cycle. This is the
number of days from the first day of your menstral bleeding to
the day before your next bleeding begins again. Please refer
to the chart below to determine when you should start testing.
If your cycle is shorter than 21 days or longer than 38 days,
consult your doctor. If you do not know your cycle length, you
may begin the test 11 days after your first period since the
average cycle length is 28 days. Perform 1 test each day over
a 5 day period or until the LH surge has been
detected.
Your Cycle Length Start To Test
On
21
Days
Day 6
22
Days
Day 6
23
Days
Day 7
24
Days
Day 7
25
Days
Day 8
26
Days
Day 9
27
Days
Day 10
28
Days
Day
11
29
Days
Day 12
30
Days
Day 13
31
Days Day
14
32
Days
Day 15
33
Days
Day 16
34
Days
Day 17
35
Days
Day 18
36
Days
Day 19
37
Days
Day 20
38
Days
Day 21
URINE
COLLECTION
1. Do not use
first morning urine samples as LH is synthesized in your body
early in the morning. It will not show up in your urine until
later in the day.
2. The
best time to collect your urine is between 2pm - 8pm.
3. Collect urine
at about the same time each day.
4. Reduce
liquid intake about 2 hours before collecting your urine as a
diluted urine sample can prevent the test from detecting LH
surge.
TEST PROCEDURE
STEP
1
Bring
the test pouch to room temperature (18-30°C). Collect urine
into a clean container (such as plastic cup). The best way to
collect sample is by placing a cup in the middle of urination
process. To begin testing, open the sealed pouch by tearing
along the notch. Remove the test from the pouch when you are
ready to use it.
STEP
2
Immerse the strip
into the urine with the arrow end pointing towards the urine.
Do not immerse past the MAX (maximum) line. Take the strip out
after 5 seconds and lay the strip flat on a clean, dry,
non-absorbent surface (e.g., mouth of the urine container). Do
not immerse for longer than 7 seconds.
STEP 3
Wait for colored bands to appear. Depending on the
concentration of LH in the test specimen, positive results may
be observed in as short as 40 seconds. However, to confirm
negative results, the complete reaction time (10 minutes) is
required. Do not read the results after 30 minutes as this
type of test is designed for rapid determination
only.
No LH
Surge:
Only one color band appears on the control region or
the test band is present but lighter in color intensity than
the control band. There is no LH surge even if two lines
present - as long as test line is fainter than the control
line the result is negative.
LH
Surge:
If two color bands
are visible and the test band is equal to or darker than the
control band, one will probably ovulate in the next 24-48
hours. If one wants to be pregnant, the best time to have
intercourse is after 24 hours but before 48 hours.
Invalid - Uncertain
Results:
No visible bands in the control and test
regions. Make sure to follow the above specified instructions
for optimum results.
Test results may
vary for different individuals depending on the concentration
of LH your body produces, as well as monitoring frequency, and
testing technique. The best method is to test once a day,
preferably later in the afternoon or early
evening.
Precautions
1. For single in
vitro diagnostic use only - use test only once for urine
screening.
2. Do not use test
kit beyond the expiration date.
3. Keep away from
moisture, direct sunlight, and children.
Note: If you
suspect that you have ovulation problems, or problems with
detecting LH surge, please see your physician. There are
numerous stimulatory procedures that can induce the ovulation
surge. | 
